Publications

Pawlowic lab Papers

Mattie Christine Pawlowic on PubMed and Google Scholar

*corresponding authors

Sebastian Shaw, Adam Sateriale, Mattie C. Pawlowic, Sumiti Vinayak, Carrie F. Brooks, Jessica H. Byerly, Boris Striepen* (2026). Genetic Manipulation of Cryptosporidium parvum. In: Mead, J.R., Arrowood, M.J. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2978. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-4824-7_13

Ross Bacchetti, Sarah Stevens, Leandro Lemgruber, Mariana Azevedo Gonzalez Oliva, Emma M Sands, Konstantinos Alexandrou, Michele Tinti, Lee Robinson, Jack C Hanna, Simona Seizova, Peyton Goddard, Massimo Vassalli, Mattie Christine Pawlowic*. Cryptosporidium Oocyst Wall Proteins are true components of the oocyst wall and COWP8 is not required for parasite transmission. PLoS Pathog. 2025 Oct 23;21(10):e1013561. doi: 10.1371/journal.ppat.1013561. PMID: 41129551.

Amanpreet Singh Chawla, Olivia J James, Purbasha Bhattacharya, Dina Dikovskaya, Suzanne Hodge, Lee Robinson, Mattie C Pawlowic, Mahima Swamy*. Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens. bioRxiv 2025.05.14.654011; doi: https://doi.org/10.1101/2025.05.14.654011

Peyton Goddard, Thomas Tzelos, Beatrice L Colon, Paul M Bartley, Lee Robinson, Leandro Lemgruber, Michele Tinti, Grant MJ Hall, Sarah Stevens, Louise Gibbard, Robert Bernard, George Tytler, David Smith*, Frank Katzer*, Mattie Christine Pawlowic* Tissue atlas of Cryptosporidium parvum infection reveals contrasts between the natural neonatal calf model and laboratory mouse models. bioRxiv 2025.05.05.652177; doi: https://doi.org/10.1101/2025.05.05.652177

Caldwell N, Peet C, Miller P, Colon BL, Taylor MG, Cocco M, Dawson A, Lukac I, Teixeira JE, Robinson L, Frame L, Seizova S, Damerow S, Tamaki F, Post J, Riley J, Mutter N, Hanna JC, Ferguson L, Hu X, Tinti M, Forte B, Norcross NR, Campbell PS, Svensen N, Caldwell FC, Jansen C, Postis V, Read KD*, Huston CD*, Gilbert IH*, Baragaña B*, Pawlowic MC*. Cryptosporidium lysyl-tRNA synthetase inhibitors define the interplay between solubility and permeability required to achieve efficacy. Sci Transl Med. 2024 Oct 23;16(770):eadm8631. doi: 10.1126/scitranslmed.adm8631. Epub 2024 Oct 23. PMID: 39441903.

Above featured in Farmers’ Weekly (HERE) and Chemical and Engineering News (HERE)

Hanna JC, Corpas-Lopez V^%, Seizova S^%, Colon BL, Bacchetti R, Hall GMJ, Sands EM, Robinson L, Baragaña B, Wyllie S*, Pawlowic MC*. Mode of action studies confirm on-target engagement of lysyl-tRNA synthetase inhibitor and lead to new selection marker for Cryptosporidium. Front Cell Infect Microbiol. 2023 Aug 4;13:1236814. doi: 10.3389/fcimb.2023.1236814. PMID: 37600947; PMCID: PMC10436570. ^%equal contribution *corresponding authors.

Gilbert IH, Vinayak S, Striepen B, Manjunatha UH, Khalil IA, Van Voorhis WC; Cryptosporidiosis Therapeutics Advocacy Group CTAG; Cryptosporidiosis Therapeutics Advocacy CTAG. Safe and effective treatments are needed for cryptosporidiosis, a truly neglected tropical disease. BMJ Glob Health. 2023 Aug;8(8):e012540. doi: 10.1136/bmjgh-2023-012540. PMID: 37541693.

Gissot M, Pawlowic MC, Modrzynska KK, Francia ME. Editorial: Molecular Basis of Stage Conversion in Apicomplexan Parasites. Front Cell Infect Microbiol. 2021 Apr 16;11:680184. doi: 10.3389/fcimb.2021.680184. PMID: 33937107; PMCID: PMC8085330.

Innes EA, Chalmers RM, Wells B, Pawlowic MC. A One Health Approach to Tackle Cryptosporidiosis. Trends Parasitol. 2020 Mar;36(3):290-303. doi: 10.1016/j.pt.2019.12.016. Epub 2020 Jan 23. PMID: 31983609; PMCID: PMC7106497.

Previous papers by Mattie

Basu S, Pawlowic MC, Hsu FF, Thomas G, Zhang K. Ethanolaminephosphate cytidylyltransferase is essential for survival, lipid homeostasis and stress tolerance in Leishmania major. PLoS Pathog. 2023 Jul 28;19(7):e1011112. doi: 10.1371/journal.ppat.1011112. PMID: 37506172; PMCID: PMC10411802.

Moitra S, Basu S, Pawlowic M, Hsu FF, Zhang K. De Novo Synthesis of Phosphatidylcholine Is Essential for the Promastigote But Not Amastigote Stage in Leishmania major. Front Cell Infect Microbiol. 2021 Mar 12;11:647870. doi: 10.3389/fcimb.2021.647870. PMID: 33777852; PMCID: PMC7996062.

Pawlowic MC, Somepalli M, Sateriale A, Herbert GT, Gibson AR, Cuny GD, Hedstrom L, Striepen B. Genetic ablation of purine salvage in Cryptosporidium parvum reveals nucleotide uptake from the host cell. Proc Natl Acad Sci USA. 2019 Oct 15; 116(42): 21160-21165. doi: 10.1073/pnas.1908239116. PMID: 31570573

Sateriale A., Pawlowic M., Vinayak S., Brooks C., Striepen B. (2020) Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In: Mead J., Arrowood M. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2052. Humana, New York, NY. PMID: 31452165

Moitra S, Pawlowic MC, Hsu F, Zhang K. Phosphatidylcholine synthesis through cholinephosphate cytidylyltransferase is dispensable in Leishmania major. Scientific Reports. 2019 May 20; 9(1): 7602. PMID: 31110206

Pawlowic MC, Vinayak S, Sateriale A, Brooks CF, Striepen B. Generating and Maintaining Transgenic Cryptosporidium parvum Curr Protoc Microbiol. 2017 Aug 11;46:20B.2.1-20B.2.32. doi: 10.1002/cpmc.33. PMID: 28800157

Pawlowic MC, Hsu FF, Moitra S, Biyani N, Zhang K. Plasmenylethanolamine synthesis in Leishmania major. Mol Microbiol. 2016 Jul; 101(2): 238-49. doi: 10.1111/mmi.13387. Epub 2016 May 6. PMID: 27062077

Vinayak S*, Pawlowic MC*, Sateriale A*, Brooks CF, Studstill CJ, Bar-Peled Y, Cipriano MJ, Striepen B. Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum. 2015 Jul 23; 523(7561): 477-80. doi: 10.1038/nature14651. PMID: 26176919 *These authors contributed equally to this work.

Above featured: Nature News and Views, Trends in Parasitology

Zhang O, Hsu FF, Xu W, Pawlowic M, Zhang K. Sphingosine kinase A is a pleiotropic and essential enzyme for Leishmania survival and virulence. Mol Microbiol. 2013 Nov; 90(3): 489-501. doi: 10.1111/mmi.12378. PMID: 23980754

Pawlowic MC, Zhang K. Leishmania parasites possess a platelet-activating factor acetylhydrolase important for virulence. Mol Biochem Parasitol. 2012 Nov; 186(1): 11-20. doi: 10.1016/j.molbiopara.2012.08.005. PMID: 22954769

Zhang O, Wilson MC, Xu W, Hsu FF, Turk J, Kuhlmann FM, Wang Y, Soong L, Key P, Beverley SM, Zhang K. Degradation of host sphingomyelin is essential for Leishmania virulence. PLoS Pathog. 2009 Dec; 5(12): e1000692. doi:10.1371/journal.ppat.1000692. PMID: 20011126

The Pawlowic lab supports open-access to our research publications. If you are unable to access any of the publications above, please contact Dr. Pawlowic and she will be happy to share (see information on ‘Contact’ page).